- Federal
- DOT · Federal
- Urine
- Oral fluid
THC and Drug Tests: Detection, Cutoffs, and What Affects Them
Short answer
A urine test does not look for THC itself — it looks for THCA, a metabolite your body produces after THC is processed. An oral fluid test looks for the parent compound instead. That is why the cutoffs are so different: 50 ng/mL screening in urine, single figures in oral fluid. How long either remains detectable depends heavily on the individual, which is why no honest source gives one number.
What matters most
- Urine tests measure THCA, a metabolite — not THC itself.
- Oral fluid tests measure the parent compound, delta-9 THC.
- DOT urine cutoffs for marijuana are 50 ng/mL screening and 15 ng/mL confirmatory.
- Detection time varies widely between individuals; a heavy user and a one-time user can differ by weeks.
- CBD products can contain enough THC to produce a positive result.
Jump to section
| Specimen | What is measured | Initial cutoff | Confirmatory cutoff |
|---|---|---|---|
| Urine — DOT (49 CFR §40.85) | THCA, a metabolite | 50 | 15 |
| Urine — HHS federal agency | THCA, a metabolite | 50 | 15 |
| Oral fluid — HHS federal agency | Delta-9 THC, the parent compound | 2–4 | 2 |
What this means for you
Rules differ by state and employer. This section describes federal requirements. Your state law or your employer’s written policy may add rules on top of them.
When the answer changes

Dr. Matthew Nuesse
DOT Certified Medical Examiner
Dr. Matthew Nuesse is a DOT Certified Medical Examiner who reviews Exploro's drug testing guides for medical and procedural accuracy.
Common questions
What does a cutoff concentration actually mean?
It is the concentration at or above which a laboratory reports a result as positive. Below it, the result is reported negative — even if a trace amount was detected. Cutoffs are a reporting threshold, not a measure of impairment.
My home test and the laboratory disagreed. Which one is right?
The laboratory. A home test is a screening device set to its own cutoff, and the FDA describes consumer tests as qualitative — they suggest whether a substance may be present, not how much. Laboratory testing is the reliable way to confirm a result.
Does the DOT drug test include fentanyl?
No. The DOT panel covers marijuana, cocaine, amphetamines, PCP and a set of opioids that does not include fentanyl. A rule to add fentanyl was proposed in September 2025 but has not taken effect, so it is not tested for on a DOT test today.
Official sources
Every factual claim above is drawn from these sources. Each was opened and checked on the date shown. Where a rule differs between agencies, the source that governs is named in the text.
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DOT ODAPC · DOT §40.85
49 CFR §40.85 — What are the drug test cutoff concentrations?
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eCFR · 49 CFR §40.85 (eCFR)
49 CFR §40.85 (current text)
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HHS · HHS Mandatory Guidelines (2026)
Mandatory Guidelines for Federal Workplace Drug Testing Programs — Authorized Testing Panels
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Federal Register · DOT NPRM — fentanyl (proposed)
NPRM — Addition of Fentanyl to the DOT Testing Panel
View official source ↗ (opens in a new tab) -
FDA · FDA — Home use tests
Drugs of Abuse Home Use Test
View official source ↗ (opens in a new tab) -
eCFR · 49 CFR Part 40 (eCFR)
49 CFR Part 40 — Procedures for Transportation Workplace Drug and Alcohol Testing Programs
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